SEMINAR

Proteomic analysis of conotoxin peptides

Abstract
The genus Conus comprise a group of marine gastropods which delivers potent venom via a harpoon-like needle in their prey. Their venom components include disulfide-rich peptides known as conotoxins, enzymes and other minor groups of conopeptides. Mass spectrometry has provided important information regarding the venom contents, identification of peptide sequences and posttranslational modifications made in the conopeptide. Proteomics studies on the venom of cone snails have provided insights on the envenomation strategy, biochemical processing of the venom and physiological roles of the other venom components. Mass spectrometry based-proteomics when combined with molecular biology tools can increase the rate of discovering new peptide sequences which are important for designing drugs.

BIOGRAPHY

2013-current Associate Professor and Program Chair of the Biological Engineering Department, Mapua Institute of Technology, Manila, Philippines
2011-2012 Post-Doctoral Fellow, Department of Biomolecular Engineering, Chung Yuan Christian University, Taoyuan Republic of China
2003-2010 Assistant Professor School of Chemical Engineering and Chemistry, Mapua Institute of Technology, Manila, Philippines

Research Field and Interest

My interest has been in the identification of conopeptides using mass spectrometry based proteomics in the venomous gastropods belonging to the genus Conus. Biochemical processing of the mature venom and the pharmacological properties of the peptides in the venom are determined for the possible design of drugs and probes for the neuromuscular system. My research has now extended in the synthesis and design of polymeric systems for possible drug and gene delivery applications.

Selected Publications

1. Yu-Ju Shih, Ching-Wei Tsai, Lemmuel L. Tayo and Yung Chang, Zwitterionic Nanocarriers for Gene Delivery, Intracellular Delivery II Fundamentals and Applications ISBN: 978-94-017-8895-3; 2014
2. Swapnil. Bhatia, Yong Kil, Beatrix Ueberheide, Brian T. Chait, Lemmuel L. Tayo, Lourdes J. Cruz, Bingwen Lu, John R. Yates, Marshall Bern. "Constrained De Novo Sequencing of Conotoxins", J Proteome Res. 2012 Jun 18
3. Swapnil Bhatia, Yong J. Kil, Beatrix Ueberheide, Brian Chait, Lemmuel L. Tayo, Lourdes J. Cruz, Bingwen Lu, John R. Yates III, and Marshall Bern, “Constrained De Novo Sequencing of Peptides with Application to Conotoxins” Research on Computational Molecular Biology LNBI 6577 pp. 16-30, 2011 ©Springer-Verlag Berlin Heidelberg 2011
4. Lemmuel L. Tayo, Bingwen Lu, Lourdes J.Cruz and John R Yates III. “Proteomic Analysis Provides Insights on Venom Processing in Conus textile Venom.” Journal of Proteome Research 2010, 9, 2292– 2301