SEMINAR

Molecular and Structural Biology of New Lipases and Proteases

Abstract
With the advent of bioinformatics databases and softwares, the studies of protein structure and functions have become critical in catalyst design and catalysis development. Thermostable (1-3) and cold-active (4) lipases were characterized and their structure determined. So far, significant structural data have been presented and the availability of structural data is the proponent of structural manipulation of enzymes. Changing a specific amino acid may result in modified thermostability (5), altered substrate specificity, changing response towards chemicals and altered enantioselectivity (6).

References
[1] Sayangku Nor Ariati Mohamad Aris, Adam Leow Thean Chor, Mohd Shukuri Mohamad Ali, Mahiran Basri, Abu Bakar Salleh, and *Raja Noor Zaliha Raja Abd. Rahman, “Crystallographic Analysis of Ground and Space Thermostable T1 Lipase Crystal Obtained via Counter Diffusion Method Approach,” BioMed Research International, vol. 2014, Article ID 904381, 8 pages, 2014. doi:10.1155/2014/904381
[2] Malihe Masomiana, Raja Noor Zaliha Raja Abd Rahmana, , Abu Bakar Sallehb, , Mahiran Basri (2013). A new thermostable and organic solvent-tolerant lipase from Aneurinibacillus thermoaerophilus strain HZ . Process Biochemistry 48 (2013) 169–175
[3] Afshin Ebrahimpour, Raja Noor Zaliha Raja Abd Rahman, Mahiran Basri and Abu Bakar Salleh (2011) High level expression and characterization of a novel thermostable, organic solvent tolerant, 1, 3-regioselective lipase from Geobacilus sp. Strain ARM. Bioresource Technology. 102 (13), 6972-6981
[4] Nor Hafizah Ahmad Kamarudin, Raja Noor Zaliha Raja Abd. Rahman, Mohd Shukuri Mohamad Ali, Thean Chor Leow, Mahiran Basri, and Abu Bakar Salleh (2014) A New Cold Adapted, Organic Solvent Stable Lipase From Mesophilic Staphylococcus epidermidis AT2. Protein J. 33:296–307
[5] Rudzanna Ruslan, Raja Noor Zaliha Raja Abd. Rahman *, Thean Chor Leow, Mohd Shukuri Mohamad Ali, Mahiran Basri, Abu Bakar Salleh (2012) Improvement of Thermal Stability via Outer-loop Ion Pair Interaction of Mutated T1 Lipase, International Journal of Molecular Sciences 13, 943-960
[6] Roswanira Abdul Wahab 1,2,*, Mahiran Basri 1,*, Mohd Basyaruddin Abdul Rahman 1,Raja Noor Zaliha Raja Abdul Rahman 3,4, Abu Bakar Salleh 3,4 and Thean Chor Leow (2012) Combination of Oxyanion Gln114 Mutation and Medium Engineering to Influence the Enantioselectivity of Thermophilic Lipase from Geobacillus zalihae. Int. J. Mol. Sci., 13, 11666-11680;

BIOGRAPHY

2013-present: Professor, Enzyme and Microbial Technology Research Centre, UPM
2006-2011: Professor, Deputy Dean (Research & Graduate Studies)
2004 -2005: Associate Professor, Head of Department
1998: Doctor of Engineering, Kyoto University, Japan

Research Fields and Interests:
Extensive work has been conducted on isolation and identification of microorganisms producing enzymes that are useful for industrial applications – namely lipases, proteases and amylases. From this point on, molecular biology techniques were adopted to enhance enzyme yield and properties, Projects towards deeper understanding of the functions and structures of the enzymes to decipher their hidden potentials were undertaken, where computer modeling and simulation are followed by molecular modification all validated by biophysical approaches. Research is undertaken on the development of optimal biocatalytic processes as well as product development, be it on the enzyme itself or the products of catalysis

Selected Publications:

Raja Noor Zaliha Raja Abd Rahman, Malihe Masomian, Adam Thean Chor Leow, Mohd Shukuri Mohamad Ali (2015) Influence of protein solution in nucleation and optimized formulation for the growth of ARM lipase crystal. Journal of Crystal Growth 426 (2015) 234–242

Raja Noor Zaliha Raja Abd Rahman 1*, Mohd. Shukuri Mohamad Ali 1, Shigeru Sugiyama 2,3, Adam Thean Chor Leow 1 ,Tsuyoshi Inoue 2 , Mahiran Basri 1, Abu Bakar Salleh 1 and Hiroyoshi Matsumura2 (2015) A Comparative Analysis of Microgravity and Earth Grown Thermostable T1 Lipase Crystals Using HDPCG Apparatus. Protein & Peptide Letters. Volume 22, Number 2, February 2015, pp. 173-179(7)

Joo Shun Tan, Sahar Abbasiliasi,Yu Kiat Lin, Mohd Shamzi Mohamed, Mohammad Rizal Kapri, Saeid Kadkhodaei, Yew Joon Tam, Raja Noor Zaliha Raja Abd. Rahman, Arbakariya B. Ariff. (2014) Primary Recovery of Thermostable Lipase 42 Derived From Recombinant Escherichia coli BL21 in Aqueous Two-Phase Flotation. Separation and Purification Technology. 133 (2014) 328–334

Velayudhan Ranjani, Stefan Janecek, Kian Piaw Chai, Shafinaz Shahir, Raja Noor Zaliha Raja Abdul Rahman, Kok Gan Chan, and Kian Mau Goh. (2014) Protein engineering of selected residues from conserved sequence regions of a novel Anoxybacillus α-amylase Sci. Rep. 4: 5850;DOI:10.1038/srep05850 (2014)

Nor Hafizah Ahmad Kamarudin, Raja Noor Zaliha Raja Abd. Rahman, Mohd Shukuri Mohamad Ali, Thean Chor Leow, Mahiran Basri, and Abu Bakar Salleh (2014) Unscrambling the effect of C-terminal tail deletion on the stability of a cold-adapted, organic solvent stable lipase from Staphylococcus epidermidis AT2", Molecular Biotechnology. Mol Biotechnol (2014) 56:747-757