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PROFESSOR DAVID OLLIS |
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The Australian National University David.Ollis@anu.edu.au |
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SEMINAR | BIOGRAPHY | ||||
Sunday 15th November | Session Three | ||||
Evolving Proteins: Bio-inspired Chemistry Abstract In addition to producing enzymes with enhanced catalytic properties, directed evolution offers a new and powerful tool with which to probe enzyme mechanics. Mutants with enhanced catalytic properties can be probed with structural tools and spectroscopic techniques to give a better understanding of how they function. This information can then be used to engineer another generation of enzymes (2). References (2) Porter, J.L., Boon, P.L.S., Murray, T.P., Huber, T.L. Collyer, C.A. and Ollis, D.L. Directed evolution of new and improved enzyme functions using and evolutionary intermediate and multi-directional search. ACS Chemical Biology (2015) 10(2):611-21. |
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2005-present: Professor, Protein Crystallography and Engineering Lab, RSC Research Interests X-ray crystallography: protein structure determination, protein structure and function studies: of replication proteins, proteins involved in nitrogen uptake in bacteria and receptor structure and function. Protein engineering with an emphasis on directed evolution. Selected publications Ollis, D. L., Brick, P., Hamelin, R., Huong, N. G. and Steitz, T. A. (1985) Structure of the Large Fragment of Escherichia coli DNA Polymerase I Complexed with dTMP. Nature, 313, 762-766. Ollis, D. L., Kline, C. and Steitz, T. A. (1985) Domain of E. coli DNA Polymerase I Shows Sequence Homology with T7 DNA Polymerase. Nature, 313, 818-819. Ollis, D. L., Cheah, E., Cygler, M., Dijkstra, B., Frolow, F., Franken, S. M., Harel, M., Remington, S. J., Silman, I., Schrag, J., Sussman, J. L., Verschueren, K. H. G. and Goldman, A. (1992) The alpha/beta Hydrolase Fold. Protein Engineering, 5, 197-211. Jaggi, R., van Heeswijk, W. C., Westerhoff, H. V., Ollis, D. L., and Vasudevan, S. G. (1997) The two opposing activities of adenylyl transferase reside in distinct homologous domains, with intramolecular signal transduction. The EMBO Journal, 16, 5562-5571. Carr, P. D., Gustin, S. E., Church, A. P., Murphy, J. M., Ford, S. C., Mann, D. A., Woltring, D. M., Walker, I., Ollis, D. L. and Young, I. G. (2001) Structure of the complete extracellular domain of the common subunit of the human GM-CSF, IL-3 and IL-5 receptors reveals a novel dimer configuration. Cell, 104, 291-300. Yang, H., Carr, P.D., Yu-McLoughlin, S., Liu, J.W., Horne, I. Qui, X., Jeffries, C.M.J., Russell, R.J., Oakeshott, J.G. and Ollis, D.L. (2003) Evolution of an organophosphate-degrading enzyme: a comparison of natural and directed evolution. Protein engineering 16(2) 135-145.
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