SEMINAR

The pre-structured motifs (PreSMos) as the active sites in intrinsically disordered proteins (IDPs)

Abstract
About one-third of the entire protein kingdom consists of “unstructured” proteins or IDPs (intrinsically disordered proteins). These proteins have broken the golden dogma in structure biology, 3-D structure = function. Furthermore, they are involved in many critical diseases such as cancers, neurodegenerative diseases (Alzheimer’s disease, Parkinson’s disease, prion diseases etc), and viral diseases. Extensive efforts to understand the fundamental mechanism of how IDPs recognize targets have revealed the presence of transient secondary structural elements in free IDPs named PreSMos that are primed to bind targets [1-3]. The PreSMos involved in various target recognition will be described.

References
[1] Lee, H., Mok, H., Muhandiram, R., Park, K.-H., Suk, J.-E., Kim, D.-H., Chang, J., Sung, Y. C., Choi, K. Y., Han, K.-H. (2000) Local structural elements in the mosrly unstructured transcriptional activation domain of Human p53. J. Biol. Chem., 275, 29426-32.
[2] Kim, D.-H., Lee, C., Cho, Y.-J., Lee S.-H., Cha E.-J., Lim, J.-E., Sabo T. M., Greisinger, C., Lee, D., Han, K.-H. (2015) A pre-structured helix in the intrinsically disordered 4EBP1. Mol BioSyst. 11, 366-9.
[3] Lee, S.-H., Kim, D.-H., Han J. J., Cha, E.-J., Lim, J.-E., Cho, Y.-J., Lee. C., Han, K.-H. (2012) Understanding pre-structured motifs (PreSMos) in intrinsically disordered proteins. Curr. Protein Pep. Sci., 13, 34-54.

BIOGRAPHY

2006-present: Professor. Bioinformatics, UST. Korea
1991-present: Senior Investigator, KRIBB, Korea.
1985-1991: Research fellow, NIH, USA
1984: PhD, Physical Chemistry, University of California Davis, USA

Research Fields and Interests:
We use NMR and other biophysical techniques to establish structure-function characterization of intrinsically disordered proteins (IDPs). IDPs are peculiar proteins that have specific functions despite the fact that they do not form 3-D structures.

Selected Publications:

Kim, D.-H., Lee, C., Cho, Y.-J., Lee S.-H., Cha E.-J., Lim, J.-E., Sabo T. M., Greisinger, C., Lee, D., Han, K.-H. (2015) A pre-structured helix in the intrinsically disordered 4EBP1. Mol BioSyst. 11, 366-9.

Lee, C., Kalmar, L., Xue, Bin., Tompa, P., Daughdrill G. W., Uversky, V. N., Han, K.-H., (2014) Contribution of proline to the pre-structuring tendency of transient helical secondary structure elements in intrinsically disordered proteins. Biochim, Biophys. Acta, 1840, 993-1003.

Szollosi, D., Horvath, T., Han, K.-H., Dokholyan, N. V., Tompa, P., Kalmar, L., Hegedus, T., (2014) Descrete molecular dynamics can predict helical prestructured motifs in disordered proteins. PLoS One, 9, e95795.

Tompa, P., Han, K.-H. (2012) Intrinsically disordered proteins, Phys. Today, 65, 64-65.

Lee, S.-H., Kim, D.-H., Han J. J., Cha, E.-J., Lim, J.-E., Cho, Y.-J., Lee. C., Han, K.-H. (2012) Understanding pre-structured motifs (PreSMos) in intrinsically disordered proteins. Curr. Protein Pep. Sci., 13, 34-54.