Physical and Theoretical Chemistry
The puzzle of how time-irreversible microscopic equations of mechanics lead to the time-irreversible macroscopic equations of thermodynamics has been a paradox since the days of Boltzmann. Boltzmann simply side-stepped this enigma by stating “as soon as one looks at bodies of such small dimension that they contain only very few molecules, the validity of this theorem [the Second Law of Thermodynamics and its description of irreversibility] must cease.” Today we can state that the Fluctuation Theorem, first proposed by Denis Evans and colleagues in 1993, is a generalized, Second-Law like theorem that bridges the microscopic and macroscopic domains and links the time-reversible and irreversible descriptions. The predictions of the FT should be relevant to many nanotechnological applications. Our work in 2003 had three major themes:
The argument of the FT is the dissipation function, which is a measure of the system’s irreversibility. It was originally defined for deterministic, Newtonian equations of motion or “detailed balance”, but has been applied inconsistently to systems where the relevant time/length scales are too large to be simulated using Newtonian dynamics. In 2003 we presented a definition for a stochastically-derived dissipation function for systems described by stochastic, Langevin dynamics. We demonstrated this stochastically-derived dissipation function in experimental analyses and showed that, for a given system, there can be “different” forms of the dissipation function that obey the FT. (with J.C. Reid, D.M. Carberry, G.M. Wang, D.J. Searles [(Griffith U.], and D.J. Evans)
An illustration of a set of neighbouring Newtonian trajectories
initiated in a volume element δV (top tube) and corresponding
set of
anti-trajectories initiated in δV* (lower tube) in
coordinate-momentum (q,p) and time, t, space. For every
trajectory Γ(t) initiated
in δ, there exists a
time-reversed or anti-trajectory Γ(t)* that is initiated
in δV*. The ratio of the probability of observing trajectories
initiated within δV to those in δV* is a measure of the
system’s irreversibility.
We have shown that the FT implies that the Kawasaki function is equal to unity. More importantly, we demonstrated that deviation of the Kawasaki function from unity can be used as a diagnostic tool in simulation and experiment, indicating the quality of the sampling of data. (with D.M. Carberry, S.R. Williams, G.M. Wang, and D.J. Evans)
By attaching specially-coated latex beads to the ends of modified DNA, we are able to use an optical trap and micropipette to stretch a single bead-DNA-bead assembly and to construct a force versus extension profiles of several DNA systems. These force profiles are used to study the effect of different salts and the interactions of specific binding proteins on DNA. In 2003 and in conjunction with the Protein Synthesis and Evolution Group headed by Nick Dixon, we focused upon different end-tethering techniques so that we can efficiently stretch ds-DNA where one strand is linked to the beads, and where both strands are linked to the beads. (with G.M. Wang, D.M. Carberry, P. Schaeffer, and N.E. Dixon)